The matatu was on its way to the city centre when the driver lost control of the vehicle.
There were no casualties in the horrendous accident that left the vehicle written off.
A few passengers in the matatu sustained injuries and were rushed to hospital for treatment.
Witnesses claim the driver of the matatu applied emergency breaks as he was on speed when he tried to avoid knocking another vehicle ahead of him.
This led to the matatu overturning and rolling on the road several times.
The latest comes days after over 10 people were killed in Kisii through different road accidents.
In one incident, an overloaded Toyota Probox ferrying passengers lost control and rammed into a personal car along the Kisii-Nyamira road. Five people died on the spot.
Early on, a Premium shuttle plying the Kisii-Kisumu Highway killed five people when the driver lost control after hitting a pothole near Kisii town.